A novel, precise, accurate, rapid and cost effective isocratic reversed-phase high performance liquid chromatographic/ultraviolet (RP-HPLC/UV) method was developed, optimized and validated for estimation of Piperaquine phosphate. Effect of different experimental parameters and various particulate columns on the analysis of these analytes was evaluated. PQP was determined by using Acetonitrile, 0.1mol L-1 phosphate buffer, 1 ml triethylamine (pH-2.5 is adjusted with o-phosphoric acid) as a mobile phase at a flow rate of 1 ml/min and wavelength of 322 nm. The calibration curves were linear over the concentration ranges of 10-80 μg/ml. The limit of detection (LOD) and limit of quantification (LOQ) for PQP were 0.0323 μg/ml and 0.0981 μg/ml respectively. The analyte was eluted in less than 5.0 min. The proposed methods have been applied successfully to the analysis of cited drug either in pure form or in pharmaceutical formulations with good accuracy and precision. The method herein described can be employed for quality control and routine analysis of drugs in pharmaceutical formulations.
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